Journal: Immune Network

Article Title: Terminally Differentiating Eosinophils Express Neutrophil Primary Granule Proteins as well as Eosinophil-specific Granule Proteins in a Temporal Manner

doi: 10.4110/in.2017.17.6.410

Figure Lengend Snippet: Western blot analysis of neutrophil-specific granule proteins and ESGPs. (A) CTSG, MPO, Olig2, and MBP1 proteins were analyzed in differentiating CB cells at 6, 12, 18, and 24 days. This result is representative of cultures of CB cells from 3 donors. (B) CTSG, MPO, and MBP1 proteins were evaluated in PB eosinophils (E) and neutrophils (N) purified from 3 donors. The anti-MPO Ab used cross-reacted weakly with EPX.

Article Snippet: Blots were probed with anti-CTSG (Abcam, Cambridge, MA, USA), anti-MPO (R&D Systems, Minneapolis, MN, USA), anti-MBP1 (Abcam), anti-oligodendrocyte transcription factor 2 (Olig2) (IBL International, Hamburg, Germany), and anti-GAPDH (Santa Cruz Biotechnology, Santa Cruz, CA, USA) antibodies, followed by incubation with anti-rabbit or anti-mouse horseradish peroxidase (HRP)-conjugated secondary Ab (Cell Signaling Technology, Beverly, MA, USA).

Techniques: Western Blot, Purification

Journal: Journal of Neuroinflammation

Article Title: Silencing of Abcc8 or inhibition of newly upregulated Sur1-Trpm4 reduce inflammation and disease progression in experimental autoimmune encephalomyelitis

doi: 10.1186/s12974-015-0432-3

Figure Lengend Snippet: Glibenclamide and Abcc8 −/− promote remyelination in EAE. a – d White matter of lumbar spinal cord sections from WT control ( a ), untreated pid-30 WT/EAE ( b ), glibenclamide-treated pid-30 WT/EAE ( c ), and pid-30 Abcc8 −/−/EAE ( d ) mice, stained with Luxol fast blue (LFB) or immunolabeled for MBP (myelin), CNPase, Olig2 (oligodendrocytes), or PDGFR-α (oligodendrocyte progenitor cells (OPC)), as indicated; nuclei stained with DAPI in the MBP sections; original magnification, ×200 (LFB) or ×400 (all immunolabelings). e three left panels : Percent of quadrants with myelin loss by LFB staining, by MBP immunolabeling, or by CNPase immunolabeling. e two right panels : Quantification of Olig-2-expressing cells in white matter or of PDGFR-α-expressing OPC near the central canal; four mice/group; ## P < 0.01 and ### P < 0.001 with respect to WT control; *** P < 0.001 with respect to WT/EAE; scale bars, 100 μm

Article Snippet: Primary antibodies were directed against the following: CD45 (1:1500; #ab10558; Abcam, Cambridge, MA); CD3 (1:200; #ab5690; Abcam); CD20 (1:100; #sc-7735; Santa Cruz Biotechnology, Santa Cruz, CA); CD11b (1:800; #NB110-89474; Novus Biologicals, Littleton, CO); TNF-α (1:500; #sc-1350; Santa Cruz Biotechnology); IFN-γ (1:100; #bs-0480R; Bioss, Woburn, MA); IL-17 (1:50; #sc-7927; Santa Cruz Biotechnology); IL-10 (1:50; #sc-1783; Santa Cruz Biotechnology); MBP (1:500; #ab40390; Abcam); CNPase (1:1000; #MAB326; EMD Millipore, Billerica, MA); Olig-2 (1:2000; #MABN50; EMD Millipore); PDGFR-α (1:500; #sc-338; Santa Cruz Biotechnology); and SMI-312 (1:1000; #SMI-312R; Covance Inc., Gaithersburg, MD).

Techniques: Staining, Immunolabeling, Expressing